Fig. 5: Mini-Ph condensates recruit PRC1 from extracts. | Nature Communications

Fig. 5: Mini-Ph condensates recruit PRC1 from extracts.

From: Phase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activity

Fig. 5: Mini-Ph condensates recruit PRC1 from extracts.The alternative text for this image may have been generated using AI.

a Scheme for isolating Mini-Ph-chromatin condensates from nuclear extracts. b Representative images of condensates formed in each of the four indicated reactions. c Quantification of phase-separated condensates (% area covered by condensates, nine images analyzed for each of three experiments using YOYO-1 staining). p Values are for one-way ANOVA with Tukey’s correction for multiple comparisons. d Ratio of average intensity in condensates formed by Mini-Ph + chromatin + nuclear extracts (reaction 3) vs. Mini-Ph + chromatin (reaction 4) for 3 experiments. p Value is for one-sample t test comparing the ratio to the expected value of 1. e SYBR Gold stained gel of nucleic acid content of pelleted reactions. Reactions 1–4 are as indicated in panel B for (c–i). Summary of three experiments quantifying the fraction of chromatin (f) and RNA (g) in the pellet. p Values are for paired two-tailed t test between reactions 3 and 4. h Representative Western blots of one experiment analyzing the content of pelleted condensates. Equal amounts of pellet and supernatants were loaded. i Summary of three experiments analyzing the content of condensates formed in extracts. Su(Z)12 was only analyzed in two experiments. One-way ANOVA was used to compare all three samples for each antibody with Tukey’s multiple-comparison test. All bar graphs show mean, and error bars are SEM. See also Supplementary Fig. 15.

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