Fig. 5: Ethanol does not impair the responsiveness of BG to NE.

a Upper, Visualization of the difference between responses to ATP (100 μM) and NE (30 μM) of BG in acute cerebellar slices from 4-week-old Aldh1l1-CreER^T2;Ai95 mice. Red circumferences indicate ROIs enclosing representative BG somata in the PCL. Lower, Ca²⁺ change traces for each ROI. Double-line blanks 11 min of washout with aCSF. Nine independent experimental repetitions were obtained with similar results. b Same as in a; however, NE was bath applied in the presence of ethanol (30 mM). Ten independent experimental repetitions were obtained with similar results. c Left, Population data: Comparison of peak Ca²⁺ change in individual BG in response to NE or ATP. Diagonal illustrates equi-response line. Right, Ratio of Ca²⁺ responses of individual BG to NE/ATP. Red symbols indicate mean ± SEM. Control, n = 174 BG from nine slices and three mice; ethanol, n = 170 BG from 10 slices and 3 mice; unpaired, two-tailed Student’s t-test (t(342) = −0.222; n.s. not significant). EtOH ethanol. Source data are provided as a Source Data file.