Fig. 8: Ethanol inhibition of vigilance-dependent Ca2+ elevations in NE terminals accounts for loss of BG Ca2+ responsiveness. | Nature Communications

Fig. 8: Ethanol inhibition of vigilance-dependent Ca2+ elevations in NE terminals accounts for loss of BG Ca2+ responsiveness.

From: Ethanol abolishes vigilance-dependent astroglia network activation in mice by inhibiting norepinephrine release

Fig. 8: Ethanol inhibition of vigilance-dependent Ca2+ elevations in NE terminals accounts for loss of BG Ca2+ responsiveness.

a Confocal image of 6-week-old Dbh-Cre;Lck-GCaMP6fflox mouse LC stained for eGFP (Lck-GCaMP6f, green) and dopamine β-hydroxylase (Dbh) (magenta). Three independent experimental repetitions were obtained with similar results. b Upper, In vivo image of Dbh-Cre;Lck-GCaMP6fflox mouse cerebellar molecular layer. Lower, Locations of ROIs. Nineteen independent experimental repetitions were obtained with similar results. c Upper, Pseudocolour plot of ROIs’ Ca2+ dynamics. Green bars, enforced locomotion. Lower, Average Ca2+ response of all ROIs (dark red) and Ca2+ response traces within number-defined ROIs. d Respective pairwise linear Pearson correlation coefficient plots between individual ROIs’ Ca2+ change traces during indicated experimental episodes. e–g Population data representing mean ΔF/F10s (e), mean time to peak (f), and mean correlation coefficient (r) (g) among ΔF/F traces of individual ROIs. Red symbols, mean ± SEM. Lines between dots support readability. n = 10 mice; repeated measures ANOVA (e F(2, 18) = 29.575; f F(2, 18) = 27.698; g F(2, 18) = 49.194) was followed by Tukey–Kramer correction; n.s. not significant. Source data are provided as a Source Data file.

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