Table 4 Summary of the feedback loop statistics for the Tc holotoxin data.

From: TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM

Feedback round

Good classes

Good particles

Picks/Mic

Good picks/Mic

Resolution

Relative good picks

1

130

12,595

130

25

4.28

0.19

2

145

10,406

100

21

4.24

0.21

3

151

14,534

74

29

4.36

0.40

4

146

14,081

71

28

4.28

0.40

5

155

14,935

68

27

4.28

0.40

6

140

13,566

69

27

4.24

0.39

6 + T 0.194

145

13,954

55

28

4.24

0.50

  1. For every feedback round as well as the final run after optimization of the picking threshold (6 + T x.xx) the number of classes labeled “good” by Cinderella; the number of particles included in these classes; the total number and the number of good particles picked per micrograph; the final resolution of the 3D reconstruction; and the relative amount of good particles (highlighted in bold) are listed for 500 micrographs.
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