Fig. 5: Pli1-dependent SUMO chain promotes relocation to NPCs but are toxic to RDR.

a Sensitivity of strains to indicated genotoxic drugs. Ten-fold serial dilution of exponential cultures were dropped on appropriate plates as described in Fig. 4a. b Co-localization event in S-phase cells in indicated conditions and strains as on Fig. 2a. p value was calculated by Fisher’s exact test for OFF and ON groups for each mutant and condition. c Binding of Npp106-GFP to the RFB in indicated strains. Upstream and downstream distances from the RFB are presented in bp. Primers targeting ade6 gene were used as unrelated control locus. Values are mean of three independent biological repeats ± SD. p value was calculated using two-sided t-test. d MSD of the RFB in OFF and ON conditions in S phase cells of pli1Δ mutant over indicated time interval (Δt) calculated for n independent cells, as described on Fig. 1d. Black bars correspond to SEM. e Frequency of RFB-induced Ura⁺ reversion in indicated strains and conditions. Each dot represents one sample from eight independent biological replicate for each strain. Bars indicate mean values ± SD. p value was calculated by two-sided t-test. f Co-localization event in S-phase cells in indicated conditions and strains as in Fig. 2a. p value was calculated by Fisher’s exact test for OFF and ON groups for each mutant and condition. g Frequency of RFB-induced Ura⁺ reversion in indicated strains and conditions. Each dot represents one sample from n independent biological replicate for each strain. Bars indicate mean values  ± SD. p value was calculated by two-sided t-test.