Fig. 7: Pli1 safeguards fork-integrity by limiting resection of nascent strands. | Nature Communications

Fig. 7: Pli1 safeguards fork-integrity by limiting resection of nascent strands.

From: The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal

Fig. 7: Pli1 safeguards fork-integrity by limiting resection of nascent strands.The alternative text for this image may have been generated using AI.

a Representative RI analysis in indicated strains and conditions as described on Fig. 3. b Quantification of resected forks. Values are mean of four independent biological replicates ±SD. p value was calculated by two-sided t-test. c Binding of RPA (Ssb3-YFP) to the RFB in indicated strains. ChIP-qPCR results are presented as ON/OFF ratio for each mutant. Upstream and downstream distances from the RFB are presented in bp. Values are mean from four independent biological replicates ±SD. p value was calculated by two-sided t-test. Primers targeting ade6 gene were used as unrelated control locus. d SUMO-based regulation of relocation of remodeled forks to NPCs to promote RDR. See text for explanations.

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