Fig. 2: SGN morphology varies with cell body position.
From: Neuronal processes and glial precursors form a scaffold for wiring the developing mouse cochlea
a A top-down view of a wholemount cochlea from an E14.5 Neurog1CreERT2;Ai14 animal, where a random subset of SGNs express tdTomato (red). SGN morphology was analyzed at three positions in the ganglion: close to the border (orange), in the middle (green), or in the rear (purple). b A high-power view of the boxed area in (a), with reconstructions of individual SGNs shown in (b′) (XY view) and (b″) (YZ view). SGNs are color-coded according to the position of their cell body. 7 cochleae were stained and analyzed (see Supp. Figs. 1 and 2). c–e Quantification of SGN morphology. Border cells (n = 28) (orange dots) extend processes that are less directional (c), shorter (d), and have a greater slope (e) than rear cells (n = 71). Middle cells (n = 52) exhibit intermediate morphologies. Plots show raw data with means ± SEM superimposed. Directionality: border cells: 0.803 ± 0.012; middle cells: 0.850 ± 0.011; rear cells: 0.903 ± 0.006. Length: border cells: 36.68 ± 2.433; middle cells: 50.88 ± 2.247; rear cells: 68.22 ± 2.418. Slope: border cells: 0.316 ± 0.037; middle cells: 0.240 ± 0.026; rear cells: 0.168 ± 0.014. Significance was assessed using ANOVA with Tukey’s multiple comparison test. Directionality: border vs middle, P = 0.188; middle vs rear, P = 0.0003; border vs rear, P < 0.0001. Length: border vs middle, P = 0.0025; middle vs rear, P < 0.0001; border vs rear, P < 0.0001. Slope: border vs middle, P = 0.107; middle vs rear, P = 0.0384; border vs rear, P = 0.0002. Source data are provided as a Source data file. Scale bars: 50 µm (a) and 25 µm (b). See Supplementary Movie 1.
