Table 1 Data collection and refinement statistics.
From: A tunable LIC1-adaptor interaction modulates dynein activity in a cargo-specific manner
Data collection (Se-Met derivative) | BICD2–LIC1 complexa | CRACR2a-LIC1 complexa |
|---|---|---|
Wavelength (Å) | 0.9775 | 0.9793 |
X-ray source | MacCHESS beamline F1 | SSRL beamline 14-1 |
Space group | P 21 21 21 | P 21 |
Cell dimensions | ||
a, b, c (Å) | 54.77, 61.84, 81.68 | 57.29, 161.99, 56.90 |
α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 104.01, 90.0 |
Resolution (Å) | 49.04–2.40 (2.49–2.40)b | 43.35–2.66 (2.78–2.66) |
Rmerge | 0.109 (0.785) | 0.359 (0.953) |
Rpim | 0.033 (0.289) | 0.114 (0.330) |
I / σ(I) | 12.0 (1.8) | 7.2 (3.0) |
No. of reflections | 218993 (8766) | 303250 (27481) |
No. of unique reflections | 19602 (1396) | 28181 (2798) |
Completeness (%) | 94.9 (67.1) | 99.9 (100.0) |
Redundancy | 11.2 (6.3) | 10.8 (9.8) |
Wilson B-factor (Å2) | 35.8 | 33.3 |
CC1/2 | 0.99 (0.76) | 0.96 (0.44) |
Effective resolutionc | ||
Overall d_min (Å) | 2.40 | 2.50 |
d_min along a*, b*, c* (Å) | 2.88, 2.57, 2.47 | 2.52, 3.00, 2.50 |
Refinement | ||
Resolution (Å) | 34.08–2.40 (2.49–2.40) | 43.35–2.66 (2.73–2.66) |
No. of unique reflections | 14,423 (907) | 23,908 (980) |
Completeness (%)d | 69.2 (23.0) | 94.5 (50.6) |
Rwork/Rfree (%) | 19.2 (26.8)/24.6 (32.5) | 19.7 (25.5)/23.2 (30.6) |
No. of atoms | ||
Protein | 1309 | 5543 |
Ca2+ | – | 8 |
Water | 42 | 49 |
B-factors (Å2) | ||
Protein | 49.8 | 63.8 |
Ca2+ | – | 49.5 |
Water | 38.1 | 42.1 |
R.m.s. deviations | ||
Bond lengths (Å) | 0.008 | 0.017 |
Bond angles (°) | 0.98 | 1.50 |
Ramachandran (%) | ||
Favored | 96.0 | 95.60 |
Outliers | 0.6 | 0.0 |
PDB code | 6PSE | 6PSD |
