Fig. 5: Loss of Gm15441 potentiates inflammasome activation by WY-14643. | Nature Communications

Fig. 5: Loss of Gm15441 potentiates inflammasome activation by WY-14643.

From: Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to PPARA agonism and fasting

Fig. 5: Loss of Gm15441 potentiates inflammasome activation by WY-14643.The alternative text for this image may have been generated using AI.

a Physiological endpoints from Gm15441+/+ and Gm15441LSL mice treated with WY-14643 for 48 h. Weight loss and liver indices (mg liver/g body mass; a measure of hepatomegaly), as well as serum ALT, AST, and glucose levels, in response to WY-14643 treatment. b Representative H&E staining from liver of Gm15441+/+ and Gm15441LSL mice treated with WY-14643 for 48 h (n = 5, 3 images/mouse). Scale bars represent 100 nm (×400). c qRT-PCR analysis of Acadm, Cyp4a14, Txnip, and Gm15441 expression in livers of mice treated with WY-14643 for 48 h. d qRT-PCR analysis of Il1b and Casp1 mRNA in livers of mice treated with WY-14643. e Western blot analysis of TXNIP, ACTB, CASP1 (cleaved), and IL1B (cleaved) protein in livers from mice treated with WY-14643 for 48 h. f Densitometric analysis of TXNIP, CASP1 (cleaved), and IL1B (cleaved) protein levels (n = 3). Each data bar represents mean ± SD for n = 5 liver samples; red bars, WY-14643 treatment. Adjusted p values, provided in the panels, as determined by ANOVA with Tukey’s multiple comparison correction, two-sided for comparisons between WY-14643 and control livers of the same genotype, or as shown (dashed horizontal lines).

Back to article page