Fig. 5: Contribution of Wnt signaling to functional compensation of hepatocytes.
From: Functional compensation precedes recovery of tissue mass following acute liver injury
Wnt target gene expression score over cycling cells (CC) and non-cycling cells (NC) from A24 and PH48 (a) and hepatocytes grouped by treatment condition (UT, A6, and P3) (b). c Wnt knockout mouse models. d Hepatocyte marker expression (Alb and Arg1) in untreated, P24, and A24 for wild type (Control), endothelial cell Wntless KO (EC-Wls), and macrophage Wntless KO (Mac-Wtls) by smFISH. Representative image taken from three independent liver lobules. Scale bar is 100 μm. (e) Average RNA expression of hepatocyte markers (Alb, Arg1, Cyp2e1, and Glul) in untreated, P24 hr, and A24 for WT, EC-Wls, and Mac-Wtls by smFISH. smFISH quantification shown as bar plot with individual replicates presented as dots (n = 3). Error bars represent s.e.m., P < 0.05 (*), < 0.005 (**), < 0.0005 (***), and < 0.0001 (****) calculated using unpaired t tests with Welch’s correction (two-tailed). Source data provided as a Source Data file.
