Fig. 6: Inhibition of HRP/ABTS and SmAA10A/CNW by ScAA10C /Avicel. | Nature Communications

Fig. 6: Inhibition of HRP/ABTS and SmAA10A/CNW by ScAA10C /Avicel.

From: Kinetic insights into the peroxygenase activity of cellulose-active lytic polysaccharide monooxygenases (LPMOs)

Fig. 6: Inhibition of HRP/ABTS and SmAA10A/CNW by ScAA10C /Avicel.The alternative text for this image may have been generated using AI.

All experiments were made in Bis-Tris buffer (50 mM, pH 6.1) at 25 °C and contained, AscA (0.1 mM), Avicel (100 g L−1) and glucose (10 mM). a Progress curves for the oxidation of ABTS (0.2 mM) by HRP (1.0 nM) (measured as consumption of AscA) in the presence of ScAA10C at different concentrations. Reactions were provided with GO (0.035 g L−1). Solid lines show linear regression of the data. The slopes of the lines correspond to the rate of the reference reaction (vref). b Dependency of vref/Vlim on [ScAA10C]. The data for the HRP/ABTS reference reaction are from panel (a). The data for the SmAA10A/CNW reference reaction are from Supplementary Fig. 13. Solid lines show nonlinear regression of the data according to Eq. (5). Data are presented as average values (n = 3, independent experiments) and error bars show SD. Source data are provided as a Source Data file.

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