Table 1 Kinetic parameters of E. coli Gnd in the reductive and oxidative directions.

From: Awakening a latent carbon fixation cycle in Escherichia coli

 

kcat (s−1)

KM (mM)

Reductive carboxylation

  

  Ribulose 5-phosphate

5.9 ± 0.2

2.8 ± 0.2

  NADPH

5.2 ± 0.2

1.0 ± 0.1

  CO2

4.7 ± 0.2

0.9 ± 0.1

Oxidative decarboxylation

  

  6-Phosphogluconate

49 ± 1

0.035 ± 0.002

  NADP+

46 ± 1

0.012 ± 0.003

  1. Values indicate mean ± standard error. Fitted Michaelis–Menten curves are shown in Supplementary Fig. 1. See the “Methods” section for a detailed description of the kinetic characterization.
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