Table 2 List of strains and expression plasmids used in this study.
From: Awakening a latent carbon fixation cycle in Escherichia coli
Name | Genotype | Source |
|---|---|---|
Plasmid pGED | pZ-ASS-gnd-eda-edd-CmR (GenBank accession MW059023) | This study |
Plasmid pG | pZ-ASS-gnd-CmR | This study |
Plasmid pED | pZ-ASS-eda-edd-CmR | This study |
DH5α | F - endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZ∆M15 ∆(lacZYA-argF)U169, hsdR17(rK - mK+), λ− | Lab collection |
MG1655 | E.coli K-12 F−, λ−, rph-1 | Lab collection |
SIJ488 | MG1655 Tn7::para-exo-beta-gam; prha-FLP; xylSpm-IsceI | |
∆rpe donor | BW25113 ∆rpe::KmR | Keio collection82 |
∆rpe | SIJ488 ∆rpe | This study |
∆tktAB | SIJ488 ∆tktA ∆tktB | |
∆tktAB+pGED mutated | SIJ488 ∆tktA ∆tktB; pZ-ASS-gnd-eda-edd-CmR; Insertion of mobile element (IS5) 104 bp upstream of pntA ORF | This study |
∆tktAB S-pntAB | SIJ488 ∆tktA ∆tktB; pntAB-Prom::CmR-Prom-‘pgi#20’-rbs‘C’ (see “Methods” section) | This study |
∆sthA donor | BW25113 ∆sthA::KmR (synonym: udhA) | Keio collection82 |
∆tktAB ∆sth | SIJ488 ∆tktA ∆tktB ∆sthA | This study |
∆tktAB W-pntAB | SIJ488 ∆tktA ∆tktB; pntAB-Prom::CmR-Prom‘pgi#1’-rbs‘C’ (see “Methods” section) | This study |
∆tktAB M-pntAB | SIJ488 ∆tktA ∆tktB; pntAB-Prom::CmR-Prom‘pgi#10’-rbs‘C’ (see “Methods” section) | This study |
∆tktAB ∆sth S-pntAB | SIJ488 ∆tktA ∆tktB ∆sthA; pntAB-Prom::CmR-Prom‘pgi#20’-rbs‘C’ (see “Methods” section) | This study |
∆tktAB ∆zwf | SIJ488 ∆tktA ∆tktB ∆zwf | |
∆tktAB ∆zwf S-pntAB | SIJ488 ∆tktA ∆tktB ∆zwf; pntAB-Prom::CmR-Prom‘pgi#20’-rbs‘C’ (see “Methods” section) | This study |
ΔpfkAB Δzwf ΔfsaAB ∆fruK (∆PZF) | SIJ488 ΔpfkA ΔpfkB Δzwf ΔfsaA ΔfsaB ΔfruK::KmR | This study |
∆PZF+pGED – mutated strains | SIJ488 ΔpfkA ΔpfkB Δzwf ΔfsaA ΔfsaB ΔfruK::KmR – various mutations (see Supplementary Table 1) | This study |
