Fig. 3: Reduced target dissociation of AGO2 germline mutants.
From: Germline AGO2 mutations impair RNA interference and human neurological development
a 293 cells were transfected with F/H-tagged AGO2. After immunoprecipitation, phosphorylation of S824 alone, of the S824–S834 cluster and of S387 was measured by a targeted quantitative mass spectrometry approach (Selected reaction monitoring with isotopically labeled spike-in peptides). The y-axis represents the percentage of individual phosphorylated peptide species assuming the sum of singly, multiply and non-phosphorylated peptides to be 100%. Significance was assessed by two-sided Student’s t test in relation to WT. n = 3 biologically independent experiments. Data are presented as mean + SD. *, **, ***p < 0.05, 0.01, 0.001, respectively. b RNA was isolated from F/H–AGO2 immunoprecipitates and analyzed by qRT-PCR using primers for the genes indicated. The significance was assessed by two-sided Student’s t test in relation to WT. n = 3 biologically independent experiments. Data are presented as mean + SD. *, **, ***p < 0.05, 0.01, 0.001, respectively. Source data are provided as a Source Data file.
