Fig. 6: Pogz-deficient mice show reduced spontaneous firing frequency of Purkinje cells while the PC intrinsic properties are unaffected. | Nature Communications

Fig. 6: Pogz-deficient mice show reduced spontaneous firing frequency of Purkinje cells while the PC intrinsic properties are unaffected.

From: Pogz deficiency leads to transcription dysregulation and impaired cerebellar activity underlying autism-like behavior in mice

Fig. 6

a Single unit activity recorded from PCs in lobules V−VI of the cerebellum. Asterisks indicate complex spikes. b, c Spontaneous firing frequency of SS (b) and CS (c) of all genotypes (n {cells/animals} = 23/5 control (blue), 32/5 cKO+/− (gray), 25/3 cKO−/− (red). d AP shape for all genotypes. In the bottom is the superpositioned normalized AP. e AP threshold of PCs of all genotypes. n = 28/3 control, 21/2 cKO+/−, 21/2 cKO−/−. f Input resistance of PCs. n = 25/3 control, 15/2 cKO+/−, 21/2 cKO−/−. g The response of PCs of all genotypes to 400 pA current pulse injections. h The average current-to-firing frequency (I − f) curve of all genotypes. Shaded area indicates SEM. Repeated-measures ANOVA; n = 28/3 control, 21/2 cKO+/−, 21/2 cKO−/−. The P values at the top of the plots are for association between the quantitative measurements and the number of intact Pogz alleles calculated with a linear regression model. Pairwise comparisons between genotypes were calculated by two-tailed t test and the significance is represented by *P < 0.05; ***P < 0.001; n.s. not significant. Quantitative data are mean ± SEM.

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