Fig. 2: Juxtaposed DMVs form membrane junctions that lead to homotypic fusion.
From: SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography
Tomograms showing DMVs in VeroE6 (a–c) and Calu3 (d–f) cells at 16 hpi. For each tomogram, a magnified area is shown below and indicated as a white square. 10 slices of the tomogram were averaged. a DMV–DMV interaction via a constricted outer membrane connection. b, c Tightly apposed membrane stack composed of four membranes (arrows) of varying curvature between two juxtaposed DMVs. d VP containing two inner vesicles with a tight membrane–membrane contact. e DMV interaction with a VP containing two inner vesicles. f VPs containing four inner vesicles with tight contact and an opening aperture formed by two stacked membranes indicated by white arrows. Scale bars: a–f 100 nm; magnified areas, 50 nm.
