Fig. 1: Constitutive expression of Fam3D in mouse gastrointestinal tract and loss of Fam3D leads to spontaneous colitis. | Nature Communications

Fig. 1: Constitutive expression of Fam3D in mouse gastrointestinal tract and loss of Fam3D leads to spontaneous colitis.

From: FAM3D is essential for colon homeostasis and host defense against inflammation associated carcinogenesis

Fig. 1: Constitutive expression of Fam3D in mouse gastrointestinal tract and loss of Fam3D leads to spontaneous colitis.The alternative text for this image may have been generated using AI.

a Fam3D mRNA expression in normal mouse tissues measured by real-time PCR. MLN: Mesenteric lymph nodes. b Mouse Fam3D protein expression in normal tissues measured by Western blot. Tissues from Fam3D−/− mice were used as a negative control. c Representative immunohistochemical staining for Fam3D in mouse colon. Brown color indicates Fam3D. Scale bar = 20 μm; Insert scale bar  = 10 μm. d Fam3D mRNA expression in colon fragments measured by real-time PCR. WC: whole colon. cEP: colonic epithelial cells. cLP: colonic lamina propria cells. cM: colonic muscles. e Mouse Fam3D protein expression in colon fragments measured by Western blot. WC: whole colon. cEP: colonic epithelial cells. cLP: colonic lamina propria cells. cM: colonic muscles. f Distal sections of colons obtained from 4 WT or Fam3D−/− mice. H&E sections showing the length of colonic crypts (11 to 20 crypts from each colon). n = 4 in each group. Scale bar = 100 μm; Insert scale bar = 50 μm. g Representative immunochemical staining of Ki67 in the distal sections of the colon from WT or Fam3D−/− mice. n = 4. Data is presented as the mean ± SEM. Scale bar = 100 μm; Insert scale bar = 50 μm. h F4/80-positive macrophages (green, left panel), CD3-positive lymphocytes (red, middle panel) or B220-positive lymphocytes (green, right panel) infiltrating the colon of WT and Fam3D−/− mice in homeostatic state. Scale bar = 50 μm. Positive cells in 10 high-powered microscopic fields (HPF) were counted in six sections from four independent mice from each group. Data is presented as the mean ± SEM per HPF. i The expression of proinflammatory cytokine genes including Il-1, Cxcl1, Cxcl2, Tnfa, and Ccl2 examined by real-time PCR, n = 7. j, k H&E and PAS/Alcian blue staining of samples from 1-year-old mice. Data is representative of one experiment repeated three independent times. Data is presented as the mean ±  SEM. Statistical significance was determined by unpaired, two-tailed Student’s t test.

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