Fig. 8: The HLB domain of Ioc3 is required for ISW1a to regulate transcription in conjunction with NuA4/SWR1.

a, b Shown are the changes in cell growth when subjected to a heat, Rapamycin (TOR signaling pathway) or 6-azauracil (6 AU) stress or b forced to use alternate carbon sources when IOC3 is mutated or deleted and YAF9 is deleted. Plates with 6 AU lack uracil and strains contained a centromeric plasmid (PRS416) with URA + marker. The assay had a minimum of three biologically independent samples with results the same as those depicted. c The heat map shows gene expression levels for wild-type (WT), ioc3∆cHLB and ioc3∆ in YAF9 or yaf9∆ background strains. Genes with over 1.5-fold change (FC) and false discovery rates (FDR) of <10% compared to wild type were identified and their Z scores plotted for wild-type and mutant ISW1a. The up (red) and down (blue) regulated genes are shown. Data are from three biologically independent samples. d A Venn diagram is shown for the overlap of genes differentially regulated in ioc3Δ versus cHLBΔ and ioc3Δyaf9Δ versus ioc3ΔcHLByaf9Δ with hypergeometric p value for the overlap being too small to be determined. In the lower half is shown the Venn diagram of the overlap of upregulated and downregulated genes for ioc3Δyaf9Δ versus ioc3ΔcHLByaf9Δ with hypergeometric p value <3.78 e−136.