Table 1 Affinities of wild-type and mutant ISW1a and Isw1 for mononucleosomes and dinucleosomes.

From: Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation

ISW1a

ΔcHLB

ISW1a

Isw1

DcHLB

Nucleosome

0N150N26

30N150N26

50N150N26

0N150N26

30N150N26

50N150N26

0N70

0N70

0N70

KD (nM)

45.9

10.2

5.29

34.5

28

12.5

30.3

21.9

32.5

95% CI

29.0–134

7.81–15.9

4.62–6.39

21.1–193

20.7–52.8

10.4–16.4

19.4–42.2

10.9–37.1

15.2-53.7

H

1.17

1.19

1.43

1.2

1.3

1.25

1.35

n.d.

n.d.

95% CI

0.921–1.45

1.01–1.39

1.22–1.67

0.812–1.66

1.01–1.64

1.02–1.50

0.895–1.96

n.d.

n.d.

R2

0.997

0.998

0.998

0.992

0.996

0.996

0.968

0.922

0.958

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