Fig. 1: Screening for functional ABE (TadA*-TadA*)-nSpCas9-DS variants.
From: Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination
a Diagram showing SpCas9 domain organization and 24 DSs (light blue lines) screened in this study. b Locations of 24 DSs and N-/C-termini in the structure of SpCas9:sgRNA:DNA complex (PDB:5F9R). c Base editing activities of ABE (TadA*-TadA*)-nSpCas9-DS variants against sgRNA-1. Adenines in sgRNA-1 were labeled as red. A–G conversion frequencies at every adenine nucleotide in 20 bp protospacer were quantified with EditR and shown in heat map. Two independent experiments were performed and editing frequencies >0.20 were labeled in the heat map. d Locations of active DSs for ABE variants and N-/C-termini in the structure of SpCas9:sgRNA:DNA complex (PDB:5F9R). DS895, DS1010 and C-Ter were shown in italic type because of lower activities of derived ABE variants as compared to other DSs. Source data are available in the Source data file.
