Fig. 3: RNA off-target activities of functional ABE (TadA*-TadA*)-nSpCas9-DS variants and N-terminal counterpart.
From: Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination
a Transcriptome analysis showing the number of edited adenine nucleotides in HEK293T cells transfected with functional ABE (TadA*-TadA*)-nSpCas9-DS variants or N-terminal counterpart and sgRNA-1. (n = 3 biologically independent samples for GFP and data here are represented as mean with S.E.M., n = 2 biologically independent samples for N-TadA*-TadA* and n = 1 for other samples). b Representative jitter plots displaying the RNA A-to-I conversion frequencies at transcriptome level in HEK293T cells transfected with functional ABE (TadA*-TadA*)-nSpCas9-DS variants or N-terminal counterpart and sgRNA-1. Source data are available in the Source data file.
