Fig. 5: Comprehensive analysis of editing windows and sequence preference for 770- and 801-TadA* (LOF)-TadA* (V82G).
From: Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination
a, b A–G conversion frequencies at each adenine position across 20 sgRNA sites were quantified and summarized for 770- TadA* (LOF)-TadA* (V82G) (a) and 801-TadA* (LOF)-TadA* (V82G) (b). Dot plots in the figure are averages of n = 2 biologically independent experiments. c, d Sequence preference was analyzed for 770- TadA* (LOF)-TadA* (V82G) (c) and 801-TadA* (LOF)-TadA* (V82G) (d). *p < 0.05, ***p < 0.001; ****p < 0.0001 with two-tailed unpaired t-test. Dot plots in the figure are averages of n = 2 biologically independent experiments. Data here are represented as mean with S.E.M. of all dot plots in the same column. (For 770- TadA* (LOF)-TadA* (V82G), AA vs TA, p = 0.0004; CA vs GA, p = 0.0287; CA vs TA, p = 0.0037; GA vs TA, p < 0.0001. For 801-TadA* (LOF)-TadA* (V82G), AA vs CA, p = 0.0002; AA vs TA, p < 0.0001; CA vs GA, p = 0.0056; GA vs TA, p = 0.0001; AA vs AG, p = 0.0217; AA vs AT, p = 0.0127.) Source data are available in the Source data file.
