Table 1 Location summary of functional DSs for ABE-nSpCas-DS variants.
From: Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination
Name | Domain at DS site | Start site after functional domain | Sequence around DS site |
|---|---|---|---|
nCas9-DS535 | REC lobe | 535 | VTEGM/RKPAF |
nCas9-DS583 | REC lobe | 583 | VEISG/VEDRF |
nCas9-DS770 | RuvC-II | 770 | RENQT/TQKGQ |
nCas9-DS793 | HNH | 793 | IKELG/SQILK |
nCas9-DS801 | HNH | 801 | LKEHP/VENTQ |
nCas9-DS895 | HNH | 895 | KLITQ/RKFDN |
nCas9-DS905 | HNH | 905 | LTKAE/RGGLS |
nCas9-DS919 | HNH | 919 | AGFIK/RQLVE |
nCas9-DS1010 | RuvC-III | 1010 | ESEFV/YGDYK |
nCas9-DS1029 | RuvC-III | 1029 | KSEQE/IGKAT |
nCas9-DS1249 | CTD | 1249 | KLKGS/PEDNE |
