Fig. 3: Asymmetric assembly of the TgMcrBC complex.
From: Structural asymmetry governs the assembly and GTPase activity of McrBC restriction complexes
a, b Bottom and side views of the cryo-EM density map of the TgMcrBC half-complex. TgMcrB subunits are colored as in Fig. 1, TgMcrC is shown in orange, and nucleotides in yellow. c Slice section through the map of the TgMcrBC half-complex at the level of the bound nucleotides, indicated by the dashed line in b. Solid and empty arrowheads indicate tight and loose interfaces, respectively. d Domain architecture of TgMcrC. e Close-up view of the interactions of TgMcrC with TgMcrB at the bottom of the hexamer, indicated by the black square in a. f Close-up view of the interactions of TgMcrC with the TgMcrB hexamer at the E/F and F/A interfaces, indicated by the dashed black square in c. Dashed lines indicate hydrogen bonds.
