Fig. 2: Weak or missing density in cryo-EM map indicates disorder at the interface of membrane and matrix arm of inactive mutant F89ALYRM6. | Nature Communications

Fig. 2: Weak or missing density in cryo-EM map indicates disorder at the interface of membrane and matrix arm of inactive mutant F89ALYRM6.

From: Essential role of accessory subunit LYRM6 in the mechanism of mitochondrial complex I

Fig. 2: Weak or missing density in cryo-EM map indicates disorder at the interface of membrane and matrix arm of inactive mutant F89ALYRM6.

Superimposed models of F89ALYRM6 mutant (cyan) and WT complex I from Y. lipolytica (PDB 6RFR, gray) (superimposed at the central section of the membrane arm (subunit ND4); Fe-S cluster N2 is shown in sphere representation). Structural elements showing disorder in mutant F89ALYRM6 are highlighted in red. Inset, residues at position 89LYRM6 of subunit LYRM6 are shown in stick representation for WT and mutant. Structural elements of subunits ND1 (hot pink), ND3 (yellow), NDUFS2 (green) and accessory NDUFA9 (forest) showing disorder in mutant F89ALYRM6 are displayed in cartoon representation (density maps are shown in Supplementary Fig. 5). The arrow indicates tilting of the peripheral arm in the mutant (Supplementary Fig. 6).

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