Fig. 1: Analyses of stress sensitivity and whole-cell proteome of human Hop KO cells. | Nature Communications

Fig. 1: Analyses of stress sensitivity and whole-cell proteome of human Hop KO cells.

From: The Hsp70-Hsp90 co-chaperone Hop/Stip1 shifts the proteostatic balance from folding towards degradation

Fig. 1

a Immunoblot analysis of KO clones. b Volcano plot of the normalized fold changes of the whole-cell proteomes of WT and Hop KO cells. Proteins with VIPpred values > 1.0 are variables of interest and cutoffs of >0.4 or < −0.4 are considered significant. VIPpred, the values of the variable importance in projection (VIP) predicted components (pred) were derived as mentioned in Methods. LFQ label-free quantification value. c GO term enrichment analyses of biological processes in Hop KO cells. Blue text, stress response-related terms; red text, protein folding/refolding-related terms. d Flow cytometric quantification of cell death induced by indicated reagents (n = 3 biologically independent samples). e Flow cytometric quantification of apoptotic cells after 48 h of recovery at 37 °C after HS for 0 to 60 min; for left and right panels, n = 3 and n = 4 biologically independent samples, respectively. f Flow cytometric quantification of apoptotic cells after treatment with AZC (n = 4 biologically independent samples). For the bar graphs, data are presented as mean values ± SEM. The statistical significance between the groups was analyzed by two-tail unpaired Student’s t-tests. Source data are provided as a Source Data file.

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