Fig. 1: Nuclear m6A-interacting factors concentrate at sites of nascent viral RNA synthesis. | Nature Communications

Fig. 1: Nuclear m6A-interacting factors concentrate at sites of nascent viral RNA synthesis.

From: Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing

Fig. 1

a Abundance of cellular m6A proteins is unchanged during infection. Immunoblot showing abundance of m6A writers, readers, and putative erasers over a time-course of adenovirus infection. Viral early (E1A and DBP) and late (Hexon, Penton, and Fiber) proteins demonstrate representative kinetic classes. β-Actin is the loading control. Kilodalton size markers shown on the left. b Confocal microscopy of m6A-interacting proteins (green) in mock-infected or Ad5-infected A549 cells 18 h post-infection (hpi). DBP (magenta) is the viral DNA binding protein that marks sites of nuclear viral replication centers. The nuclear periphery is shown by a dotted white line as assessed by DAPI staining. Scale bar = 10 µm. c Confocal microscopy showing the pattern of actively transcribing RNA Polymerase II phosphorylated on serine 2 of CTD (Pol II p-Ser2, green) in mock-infected cells or relative to DBP (magenta) in infected cells. Scale bar = 10 µm. All data are representative of at least three independent experiments.

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