Table 1 Plasmids used in the study.
From: SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity
Protein | Modification | Note |
|---|---|---|
hACE2 | N-terminal Myc and C-term c9 tag (TETSQVAPA) | mouseACE2 signal seq + Myc + hACE2 residues 20-805 (NM_021804) |
hTMPRSS2 | C-terminal FLAG | Full-length (NP_005647.2) |
hFurin | C-term c9 tag | Full-length (BC012181.1) |
S1D614-Fc | C-terminal Fc | Residues 1-682; codon-optimized (YP_009724390) |
S1G614-Fc | C-terminal Fc | Residues 1-682; codon-optimized; D614G mutation |
hACE2-NN-Ig | C-terminal Fc | IGH3 signal seq + hACE2 residues 20-615 (NM_021804) with H374N and H378N mutations |
hACE2-NN-Ctag | C-terminal Ctag (-EPEA) | IGH3 signal seq + hACE2 residues 20-615 (NM_021804) with H374N/H378N mutations |
MLV gag/pol | None | Full length MLV gag and pol |
pQCXIX-eGFP | None | Full-length eGFP in retroviral vector pQCXIX |
pQCXIX-FLuc | None | Full-length firefly luciferase in pQCXIX |
S protein (D614, G614, or FKO) | C-terminal FLAG | Full-length; codon-optimized (YP_009724390) |
| Â | N-/C-term FLAG | Full-length; codon-optimized; its own signal sequence |
| Â | No tag | Full-length; codon-optimized |
M protein | None | Full-length; codon-optimized (YP_009724393) |
N protein | None | Full-length; codon-optimized (YP_009724397) |
E protein | None | Full-length; codon-optimized (YP_009724392) |
