Fig. 6: Cellular immune responses stimulated by sMVA-CoV2 vectors.
From: Development of a multi-antigenic SARS-CoV-2 vaccine candidate using a synthetic poxvirus platform
Balb/c mice (n = 5) immunized twice in a 3-week interval with 5 × 107 PFU of the single or double recombinant sMVA-CoV2 vectors derived with FPV HP1.441 (sMVA-S/N hp and sMVA-N/S hp) or TROVAC (sMVA-S/N tv, sMVA-N/S tv, sMVA-S tv, sMVA-N tv) were evaluated for SARS-CoV-2-specific cellular immune responses. Antigen-specific CD8+ (a and b) and CD4+ (c and d) T-cell responses induced by the vaccine vectors after two immunizations were evaluated by flow cytometry for IFNγ, TNFα, IL-4, and IL-10 secretion following ex vivo antigen stimulation using SARS-CoV-2 S- and N-specific peptide libraries. Due to technical issues, 1–3 animals/group were not included in the CD4/TNFα analysis in c and d. One-way ANOVA with Tukey’s multiple comparison test was used to compare % of cytokine-specific T cells between immunized mice and mock controls. Lines represent median values; *0.05 < p < 0.01, **0.01 < p < 0.001, ***0.001 < p < 0.0001, ****p < 0.0001; ns = not significant. Source data are provided as a Source Data file.
