Fig. 2: Phylogenetic analyses within MPNs.

a Evolutionary trajectories of pre-invasive and adjacent invasive MPN components. For each of the three evolutionary patterns, D1, D2, and D3 indicate three hypothetical key molecular events in trunk and branches of phylogenetic tree; green dots represent the germline and red dots represent the most recent common ancestor (MRCA) for each pair of components. In evolution mode 1 (EM1), none of driver mutations are shared and D1 and D2 indicate different private driver alterations (Supplementary Fig. 4a), and JSCH P13-2 is a representative MPN. In evolution mode 2 (EM2), MRCA harbors critical common events (D3). Private driver alterations of D2 and D1 are restricted to the pre-invasive component (EM2A; Supplementary Fig. 4b) and the invasive component (EM2B; Supplementary Fig. 4c), respectively. Phylogenetic trees of JSCH P42 and P04 are shown to represent EM2A and EM2B separately. b Mean intratumor heterogeneity (ITH) score of 50 paired pre-invasive and adjacent invasive components suggests phenotypic differences including EM1 (MPNs, n = 5), EM2A (MPNs, n = 26), and EM2B (MPNs, n = 19). The differences were assessed using Kruskal–Wallis H test. c Comparisons of ITH levels between 50 paired pre-invasive and invasive components were performed using two-sided Wilcoxon Rank-Sum test. The box plot displays the first and third quartiles (top and bottom of the boxes), median (band inside the boxes), and lowest and highest point within 1.5 times the interquartile range of the lower and higher quartile (whiskers). Inv, Invasive and Pre-Inv, Pre-invasive.