Fig. 1: Live-cell microscopy demonstrating prolonged abnormal mitosis with subsequent formation of one endomitotic multilobulated nucleus (MLN) in cohesin depleted cells.
From: Cohesin depleted cells rebuild functional nuclear compartments after endomitosis
a Selected points from time-lapse imaging (Σt = 21 h, Δt = 15 min) of untreated control cells (− auxin) with the accomplishment of mitosis (M1) with in 1h (time 02:45–03:45) and subsequent formation of two daughter nuclei. DNA stained with SiR-DNA. A second mitosis (M2) of one daughter nucleus is shown at time 20:15. b Selected time lapse images of nuclei after cohesin degradation (+ auxin) conducted in parallel to control cells demonstrate a prolonged mitotic stage. Mitosis (M) emerges at time 6:30 after auxin treatment, transition into one abnormal multilobulated daughter nucleus (MLN) is seen 14:45 h later (time 21:15). Mitosis (M’) emerges 7 h after auxin treatment (time 07:00), transition into an MLN is seen 4:45 h later (time 11:45). c Time-lapse imaging from the same series at a higher zoom shows an aberrant mitosis with an adumbrated formation of two daughter nuclei (time 06:45), that finally appear as one MLN at time 7:15. Scale bar: 10 µm. M, M1, M2, M’ denote different mitoses. Images shown in a–c show representative nuclei from one of three independent experiments.
