Fig. 6: Maintenance, postmitotic rebuilding and de novo formation of typical replication patterns after cohesin depletion.

a–e Overlay images (left) show representative SIM sections of DAPI-stained nuclei (red) with replication domains (RDs)(green) identified by replication labeling (RL) in different stages of S-phase. RDs in the same nuclear sections are also displayed in gray (right). a Control nuclei fixed 6 h after RL with typical patterns for early, mid and late replication, respectively. b Maintenance of the same typical replication patterns in nuclei of cohesin depleted, pre-mitotic cells fixed 6 h after RL. c Cohesin depleted mitotic cell with replication labeled chromatin domains obtained under conditions as described in b. d RD patterns in individual lobuli demonstrate the ability of post-endomitotic MLN to restore RD patterns, generated by RL during the previous cell cycle. Cells were treated with auxin for 30 h after RL. e RL carried out with MLN obtained after ~30 h auxin treatment demonstrates de novo DNA synthesis with the formation of new typical replication patterns. Scale bar: 5 µm. Images shown in a–e show representative nuclei from three independent experiments.