Fig. 8: Changes in the X 3D structure after Firre deletion or inversion.

a Pearson correlated-transformed differential contact maps of the Xi at 500 kb resolution highlight differences between ΔFirre^Xi and WT, and between InvFirre^Xi and WT. The color scale shows differential Pearson correlation values, with loss and gain of contacts in the mutants versus WT appearing blue and red, respectively. See text for description of changes. b Virtual 4 C plots derived from Hi-C data at 500 kb resolution using Firre as the viewpoint on the Xi in WT (blue), ΔFirre^Xi (green) and InvFirre^Xi (gray) show an increase in contacts between Firre and Dxz4 in InvFirre^Xi. c Pearson correlated-transformed differential contact maps of the Xa and Xi at 500 kb resolution to highlight differences between ΔFirre^Xa and WT. The color scale shows differential Pearson correlation values, with loss and gain of contacts in ΔFirre^Xa versus WT appearing blue and red, respectively. See text for description of changes. d Pearson correlated-transformed contact maps (40 kb resolution) of the Xa for 4 Mb around the Firre locus highlight the loss of the strong boundary between TADs on the Xa in ΔFirre^Xa versus WT (see Supplementary Fig. 7c for corresponding maps of the Xi).