Fig. 3: Hetero-oligomerization in Eukaryotic membranes.
From: Viral Bcl2s’ transmembrane domain interact with host Bcl2 proteins to control cellular apoptosis
a–f Relative fluorescence (RFU) for the hetero-oligomerization of vBcl2 and cBcl2 TMDs. The mean and standard deviation of at least three independent experiments are shown. Solid dots represent the results of individual experiments (n ranges between 3 and 10). The TMD included in the VFP chimeras (VN or VC) is indicated below each bar. The GpA TMD homodimer was used as a positive control and as the normalization value (dotted line). The interactions of each partner in the hetero-oligomers with T20 TMD were used as negative controls (i.e., X/T20 and T20/Y for the X/Y interaction). The blue (VN/T20 TMD) and red (VC/T20 TMD) lines within each bar indicate the fluorescence of the controls. An interaction (highlighted in green) was considered only if the RFU obtained was significantly higher (two-tailed homoscedastic t-test, p-value < 0.05) than those of both negative controls. g Correlation of the BiFC assay results in HCT 116 and HEK 293T cells. Green dots indicate interactions in HEK 293T cells. A linear trend line and the corresponding coefficient of correlation are shown.
