Fig. 5: Interaction between MyxV and Bax TMDs.

a Representative example of dose–response curves used to calculate LD₅₀ values in the BlaTM assay. The TMD homodimer of GpA was used as a positive control (black), while the T20 TMD was used as a negative control (white). b Summary of the results for the BlaTM assay. The indicated βN and βC chimeras were expressed in E. coli and the resulting ampicillin LD₅₀ measured. LD₅₀ mean and standard deviation of at least three independent experiments (n ≥ 3) are shown. Two-tailed homoscedastic t-test p-values are indicated. Solid dots represent the results of individual experiments. c Predicted (PredDIMER) contact area between MyxV and Bax TMDs. The position (length and angle) of each residue in a putative α-helix is indicated. A dotted line encircles the contact area in each TMD. The hydrophobicity of the residues is shown with a color scale. d Model of a putative dimer between MyxV TMD and Bax TMD, obtained with PredDIMER. The residues involved in the interaction are indicated. Glycine and alanine residues are highlighted in bold while aromatic residues are shown in red. e BlaTM analysis for the interaction between MyxV and Bax TMDs carrying substitutions of Gly 158 (MyxV) and/or Gly 179 and Ala 183 (Bax) by Ile. LD₅₀ mean and standard deviation of at least three independent experiments are shown (n ≥ 3), solid dots represent the results of individual experiments. f Bax TMD-induced cell death. Cells were transfected with Bax TMD and H2, Bcl2 or MyxV TMD or mock-transfected; 24 hpt cell viability was measured by Trypan blue staining. Survival percentage mean and standard deviation of five independent experiments (n = 5) are shown. Two-tailed homoscedastic t-test p-values are indicated. Solid dots represent the results of individual experiments.