Table 3 Optimized gene-editing enzymes.

From: Engineering rules that minimize germline silencing of transgenes in simple extrachromosomal arrays in C. elegans

Characteristics

Optimized enzyme

Germline optimized (with PATCs)

Enzyme

CAI score

piRNA score

Gateway entry vector

Expression (Peft-3)

Gateway entry vector

Expression (Psmu-1)

RFP co-expression (Psmu-2 tagRFP)

Inducible expression (Phsp-16.41)

Mos1 transposase

0.92

0

pCFJ1470

pCFJ1477

pCFJ2471

pCFJ1532

pCFJ2475

 

Cre recombinase

0.99

0

pCFJ2118

pCFJ2139

pCFJ2126

pMDJ20

pMDJ22

pMDJ113

Enhanced Flp recombinase

0.7

1.5

pCFJ2119

pCFJ2130

pCFJ2124

pMDJ21

pMDJ23

pMDJ112

Cas9

0.98

9

pCFJ2469

 

pCFJ2470

pCFJ2477

pCFJ2474

 
  1. The genes were codon-adapted for high expression (CAI score)44, depleted of piRNA homology (piRNA score)40,45,72, and PATC-rich introns were inserted by Golden-Gate assembly.
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