Fig. 10: Chemogenetic neuronal activation increases mTOR activity. | Nature Communications

Fig. 10: Chemogenetic neuronal activation increases mTOR activity.

From: Chronic neuronal activation increases dynamic microtubules to enhance functional axon regeneration after dorsal root crush injury

Fig. 10

DRGs injected with either hM3Dq or mCherry. All animals received CNO. One day after CNO administration, sections were stained for p-S6, a marker of mTOR activation. While some mCherry+ neurons had detectable p-S6, many did not (a–c, arrowheads). However, the vast majority of hM3Dq+ DRGs had high levels of p-S6 (d–f, arrows), even neurons that weakly expressed hM3Dq (asterisks). (g) Quantification of p-S6 fluorescence intensity. N = 43 mCherry+ neurons and 42 hM3Dq+ neurons. Mean ± SEM. Two-tailed unpaired t-test, ****p < 0.0001. Scale bar: 70 µm. Source data are provided as a Source Data file.

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