Table 4 Selected reaction monitoring (SRM) parameters for determination of SARS-CoV-2 targeted peptides. DGI: DGIIWVATEGALNTPK; IGM: IGMEVTPSGTWLTYTGAIK; HSG: HSGFEDELSEVLENQSSQAELK; and SYE: SYELPDGQVITIGNER.

From: Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts

Assay

Peptide

Start time (min)

End time (min)

Precursor (m/z)

Product (m/z)

Collision energy (V)

RF Lens (V)

Tier 3

DGI (+2)

1.7

2.3

842.948

1001.526

25

160

     

1100.595

26

 
     

1286.674

25

 
 

IGM (+2)

2.2

2.6

1013.021

1394.731

31

170

     

1495.779

31

 
     

1594.847

31

 
 

HSG (heavy) (+3)

0.7

1.7

835.359

1017.462

27

170

     

1143.423

27

 
     

1243.489

27

 

Tier 1

DGI (+2)

1.0

2.0

842.948

1001.526

25

160

     

1100.595

25

 
     

1286.674

25

 
 

DGI (heavy) (+2)

1.0

2.0

852.420

1013.49

25

160

     

1113.556

25

 
     

1301.629

25

 
 

IGM (+2)

1.5

2.5

1013.021

1394.731

31

170

     

1495.779

31

 
     

1594.847

31

 
 

IGM (heavy) (+2)

1.5

2.5

1023.490

1409.687

31

170

     

1511.731

31

 
     

1611.797

31

 
 

SYE (+2)

0

1.4

895.949

901.51

25

170

     

1201.617

25

 
     

1298.67

25

 
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