Fig. 4: CE-ESI-MS analysis of Dictyostelium discoideum extract. | Nature Communications

Fig. 4: CE-ESI-MS analysis of Dictyostelium discoideum extract.

From: Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry

Fig. 4

a Separation of PP-InsP and InsP standards using a modified BGE (30 mM ammonium acetate titrated with NH4OH to pH 9.0). Enlarged inserts for PP-InsP5 (red star) and InsP5 (green star) reveal the separation of all regioisomers using the following parameters: CE voltage: 30 kV, CE current: 19 µA, injection: 50 mbar, 10 s (10 nL), solutes: 10 µM for Ins(1,3,4,5,6)P5, InsP6, 5-PP-InsP5, 1-PP-InsP5, and 1,5-(PP)2-InsP4; 20 µg/mL for Ins(1,3,4,5)P4. b PP-InsPs in D. discoideum extracts. PP-InsP regioisomers could be efficiently assigned and quantified in single runs with spiked (isotopic) standards: major 5,4/6-(PP)2-InsP4, 4/6-PP-InsP5, and 5-PP-InsP5, minor 1/3-PP-InsP5. c Mass spectra for PP-InsPs in D. discoideum in b. Three independent D. discoideum AX2 (wild type) extracts were analyzed giving identical results. Source data are provided as a Source Data file.

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