Fig. 2: Novel diversity within the collection.
From: A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity
a Phylogenomic tree of Sodaliphilus pleomorphus within members of the order Bacteroidales (phylum Bacteroidetes) together with a phase contrast micrograph of the strain grown on WCA agar with 5% sheep blood for 7 days at 37 °C under anaerobic conditions. b Microscopic investigations of Bullifex porci DSM 105750T. Picture 1 represent cells in their own growth medium observed immediately after removal from the anaerobic culture tube. The image was obtained using a N-Achroplan objective (100×/1,25 Oil Ph3 M27) mounted on an Axio Lab.A1 microscope equipped with an Axiocam 105 camera (Zeiss, Jena, Germany). Picture 2 is a scanning electron micrograph obtained as detailed in the methods section. Panel 3 shows cells stained with FM4-64 (red; membrane) and DAPI (blue; DNA) next to the same cells observed by phase contrast (left-hand side). Panel 4 shows cells imaged by transmission electron microscopy using 1.5% (first two pictures) or 0.2% (last two pictures) glutaraldehyde fixation. Multiple other images of B. porci obtained using a variety of sample preparations and microscopy techniques are available in Supplementary Figs. 8–10. c Phylogenomic tree showing the placement of B. porci among closest relatives within the family Spirochaetaceae (phylum Spirochaetes) together with the presence (black or grey boxes) or absence (white boxes) of genes involved in cell morphology and division, peptidoglycan synthesis, and cell wall formation (the entire set of genes tested is shown in Supplementary Fig. 2). Sphaerochaeta associata was not included in the analysis as no genome is yet available for this species. KO: KEGG orthology.
