Fig. 1: BDQ modulates glycolysis and the PPP.

a The percent total abundance of each labelled and unlabelled isotopologue for glutamate and α-ketoglutarate (aKG) from ¹³C₆ glucose as the ¹³C tracer. b The percent ¹³C enrichment and carbon isotopologue distribution (CID) of glycolytic intermediates and glycolytic intermediate-derived amino acids from ¹³C₆ glucose as the ¹³C tracer. c The %M + 6 species and (d) total glucose/fructose-6-phosphate and fructose-1,6-bisphosphate. Total levels of (e) ribose-5-phosphate (R-5-P) and (f) sedoheptulose-7-phosphate (S-7-P) were measured to determine the effects on the PPP. Each column represents the mean + /− standard error of the mean (SEM) of at least three replicates. One representative experiment is shown, with the experiment being repeated a minimum of two times. A two-way ANOVA and a Tukey multiple-comparison test were used for the statistical analysis. G/F-6-P glucose/fructose-6-phosphate, His - histidine, F1,6-BP - fructose-1,6-bisphosphate, snG3P - glyceraldehyde-3-phosphate, Gly - glycine, Val - valine, UT - untreated, BDQ - bedaquiline. n = 4 biologically independent samples. *P < 0.05, **P < 0.01, ****P < 0.0001.