Fig. 4: BDQ treatment leads to dysregulation of the glyoxylate shunt and reduced total glutamine. | Nature Communications

Fig. 4: BDQ treatment leads to dysregulation of the glyoxylate shunt and reduced total glutamine.

From: Bedaquiline reprograms central metabolism to reveal glycolytic vulnerability in Mycobacterium tuberculosis

Fig. 4: BDQ treatment leads to dysregulation of the glyoxylate shunt and reduced total glutamine.The alternative text for this image may have been generated using AI.

The percent abundance of M + 2 isotopologues of alpha-ketoglutarate (αKG), succinate and malate from (a) 13C6 glucose, (b) 13C2 acetate and (c) 13C3 propionate as the 13C tracer. The %13C enrichment and carbon isotopologue distribution (CID) of (d) glutamine (Gln), (f) glutamate (Glu) as well as (e) total Gln and (g) Glu levels and the (h) Glu:Gln ratio. (i) A diagram depicting bedaquiline’s (BDQ’s) effect on Gln is also shown. Mean +/− standard error of the mean (SEM) of at least three replicate extracts is indicated. One representative experiment is shown, with the experiment being repeated a minimum of two times. A two-way ANOVA and Tukey’s multiple-comparison test were used for the statistical analysis. UT - untreated. n = 4 biologically independent samples. *P < 0.05, ***P < 0.001, ****P < 0.0001.

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