Fig. 5: BDQ exposure dysregulates the MCC.

Here we show (a) the methylcitrate cycle (MCC) and (b) carbon isotopologue distribution (CID), as well as the total levels of methylisocitrate (MIC) from (c) 13C6 glucose, (d) 13C2 acetate, (e) 13C3 propionate and (f) 13CO2 as the 13C tracer. Standard error of the mean of at least three replicate extracts is indicated. One representative experiment is shown, with the experiment being repeated a minimum of two times. A two-way ANOVA and Tukey multiple-comparison test were used for the statistical analysis. Mean + /− standard error of the mean (SEM) of at least three replicate extracts is indicated. MCM - methylmalonyl-CoA mutase, UT - untreated, BDQ - bedaquiline. n = 4 biologically independent samples. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.