Fig. 3: KAT5 or HUWE1 knockdown in BRCA2-depleted cells results in PARPi resistance. | Nature Communications

Fig. 3: KAT5 or HUWE1 knockdown in BRCA2-depleted cells results in PARPi resistance.

From: Identification of regulators of poly-ADP-ribose polymerase inhibitor response through complementary CRISPR knockout and activation screens

Fig. 3: KAT5 or HUWE1 knockdown in BRCA2-depleted cells results in PARPi resistance.The alternative text for this image may have been generated using AI.

a–c Knockdown of KAT5 or HUWE1 rescues the olaparib sensitivity of BRCA2-knockdown HeLa (a), BRCA2-knockdown U2OS (b), and BRCA2-knockout DLD1 (c) cells in cellular viability assays. d, e Olaparib-induced apoptosis is rescued by KAT5 or HUWE1 depletion in BRCA2-knockdown HeLa (d) and BRCA2-knockout DLD1 (e) cells. Asterisks indicate statistical significance (t-test, two-tailed, unequal variance), compared to siBRCA2 (d) or BRCA2KO siControl (e) samples. f KAT5 or HUWE1 depletion rescues sensitivity of BRCA2-knockout HeLa cells to PARPi in a clonogenic survival assay. All graphs reflect the average of three experiments performed after 72 h of treatment, with standard deviations shown as error bars. Source data are provided as a Source Data file.

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