Fig. 5: COX-2/PGE2 blockade promotes priming of a CD8+ Tc1-mediated immune response.
From: Tipping the immunostimulatory and inhibitory DAMP balance to harness immunogenic cell death
a A schematic depiction of the experimental procedures implemented to analyze vdLN (i.e., popliteal) and PB-circulating CD8+ T cells. b Representative gating-strategy implemented to analyze/sort CD8+ T cells from vdLN and PB by flow cytometry. c Quantification of PB-circulating CD8+ T cells post-vaccination (n = 3 per treatment group). d qPCR analysis of genes associated with functionally activated Tc1-polarized CD8+ T cells. e An illustration depicting the adapted in vivo vaccination assay to interrogate vdLN CD8+ T cells. f–i Flow cytometry analysis and quantification of vdLN CD8+ T cells 5-days post-vaccination (non-dLN n = 4; vdLN n = 5 per group). PB peripheral blood, PLN popliteal lymph node. Statistics: two-tailed, two-way ANOVA-Tukey’s multiple comparisons test (c; *p = 0.387); two-tailed, one-way ANOVA (d, T-bet; p = 0.0483), (d, Tnfa; p = 0.0080), (d, Ifng; p = 0.0002), (d, Gzmb; p = 0.0379), (f, T-bet; *p = 0.0136 and **p = 0.0089), (f, IFNg; ***p = 0.0008 and ****p < 0.0001), (f, CD107a; **p = 0.0071 and ***p = 0.0004), (h, GATA3; p = 0.0115), (h, RoRyt; ****p < 0.0001), and (h, Foxp3; *p = 0.0103 and ***p = 0.0008); and where appropriate, data are presented as mean values ±SEM.
