Fig. 5: A reciprocal hemizygosity test to identify Kcnj13 evolution.

Two hybrids between D. rerio and D. aesculapii, which are hemizygous for a Kcnj13 loss-of-function mutation. a stripes are interrupted in hybrids carrying the mutant allele from D. rerio (n > 60, nick in the blue line representing the zebrafish genome). b hybrids carrying the mutant allele from D. aesculapii (n = 6, nick in the magenta line, representing the D. aesculapii genome) are indistinguishable from wild-type hybrids (Fig. 1e).