Fig. 7: Disruption of the liver-specific 12-h clock abolishes 12-h oscillation of respiratory exchange ratio (RER).
From: XBP1 links the 12-hour clock to NAFLD and regulation of membrane fluidity and lipid homeostasis
a, b The raw (a) and relative (b) real-time RER values of the indicated mice (n = 4) housed under ad libitum feeding conditions. Individual (light line) and averaged (bold line) real-time RER values of the indicated mouse strains plotted against the indicated time points. c Eigenvalue/pencil decompositions and deconvolutions of the mean RER oscillations of 12-h (upper panel) and 24-h (lower panel) oscillations. d The relative frequency histogram (left panel) and raw values (right panel) of real-time RER of the indicated mice housed under ad libitum feeding conditions (n = 4). e, f The raw (e) and relative (f) real-time energy expenditure (EE) values of the indicated mice (n = 4) housed under ad libitum feeding conditions. Individual (light line) and averaged (bold line) real-time EE values of the indicated mouse strains plotted against the indicated time points. g The ANCOVA analysis (generalized linear model, GLM) of the real-time EE in (e, f). normalized by lean mass at the indicated light, dark, and full-day conditions (n = 4). h The body composition of the indicated mice (n = 9) housed under ad libitum feeding conditions. Unpaired Student’s t-test and one-way ANOVA analysis were performed with p value indicated. i The integrated statistical analysis is shown for the indirect calorimetry experiments of the Xbp1flx/flx and AlbCre;Xbp1flx/flx mice (n = 4) housed under ad libitum feeding conditions. One-way ANOVA analysis with Tukey’s post hoc analysis was performed with p value threshold 0.05. Blue line: Xbp1 flx/flx; red line: AlbCre;Xbp1 flx/flx. Mouse RER and EE data are graphed as the mean ± SEM. *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001.
