Fig. 1: Transcription profile across the VLT and ORF63 loci during lytic VZV infection of human epithelial cells and sensory neurons. | Nature Communications

Fig. 1: Transcription profile across the VLT and ORF63 loci during lytic VZV infection of human epithelial cells and sensory neurons.

From: Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency

Fig. 1: Transcription profile across the VLT and ORF63 loci during lytic VZV infection of human epithelial cells and sensory neurons.The alternative text for this image may have been generated using AI.

a Coverage plots denoting dRNA-Seq (ARPE-19 epithelial cells, dark blue) and cDNA-Seq (human iPSC-derived sensory neurons [HSN], teal) data aligned to the top strand of the VZV genome. dRNA-Seq data is representative of two independently sequenced biological replicates with RNA extracted from lytically VZV-infected ARPE-19 cells at 4 days post infection (4 dpi). cDNA-Seq data was generated from a pool of biological replicates collected from lytically VZV-infected HSN at 14 dpi. Schematics of the major transcripts from VLT and ORF63 loci are shown in following colors: lytic VLT isoform (lytVLT, red), canonical RNA 63-1 (63, green), lytic VLT-ORF63 isoforms (lytVLT63-1/2/3, purple) and latent VLT isoform (VLT, orange). Additional canonical VZV transcription units present are shown in grey, with ORF numbers indicated. Wide boxes indicate canonical CDS domains and while thin boxes indicate UTRs, respectively. Y-axis values indicate the maximum read depth of that track. b Analysis of VLT, RNA 63 and VLT-ORF63 isoform expression by RT-qPCR analysis using the same two independent experiments in ARPE-19 cells (dark blue) and HSN (teal) for long-read sequencing. The primer locations used for RT-qPCR analysis detecting transcripts from VLT to ORF63 loci are depicted in Supplementary Fig. 1. Source data are provided as a Source Data file. ORF; open reading frame, CDS; coding sequence, UTR; untranslated region.

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