Fig. 3: Conformational change of XylE through substrate/inhibitor binding. | Nature Communications

Fig. 3: Conformational change of XylE through substrate/inhibitor binding.

From: Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter

Fig. 3: Conformational change of XylE through substrate/inhibitor binding.

a Differential deuterium uptake pattern and peptide deuterium uptake plots (peptide on TM1-TM2 loop and on TM10-TM11 loop) between a WT with substrate (xylose)-bound state and apo state, and b between D27N with substrate (xylose)-bound state and D27N state. c E206Q with substrate (xylose)-bound state and E206Q state, d E206Q&D27N with substrate (xylose)-bound state and E206Q&D27N state and e WT with substrate (xylose)-bound state and apo state. f D27N with inhibitor (glucose)-bound state and D27N state. Figures are plotted onto 3D protein structure (PDB: 4GBY). Blue and red regions indicate negative (protected) or positive (deprotected) deuterium uptake differences between the aforementioned states, respectively. SDs for each time point are plotted as error bars (n = 3).

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