Fig. 1: Single-cell transcriptomic analysis of OS lesions. | Nature Communications

Fig. 1: Single-cell transcriptomic analysis of OS lesions.

From: Single-cell RNA landscape of intratumoral heterogeneity and immunosuppressive microenvironment in advanced osteosarcoma

Fig. 1: Single-cell transcriptomic analysis of OS lesions.The alternative text for this image may have been generated using AI.

a Graphical view of the study roadmap. Single-cell suspensions were collected from OS lesions of 11 patients followed by scRNA-seq on 10× Genomics platform, respectively. A total of 100,987 qualified single cells were recovered. The peripheral blood CD3+ T cells were isolated for cytotoxicity analysis for TIGIT blocking experiments. b The t-distributed stochastic neighbor embedding (t-SNE) plot of the 11 identified main cell types in OS lesions. c Violin plots showing the normalized expression levels of eight representative canonical marker genes across the 11 clusters. d Dot plots showing the 21 signature gene expressions across the 11 cellular clusters. The size of dots represents the proportion of cells expressing the particular marker, and the spectrum of color indicates the mean expression levels of the markers (log1p transformed). e Relative proportion of each cell cluster across 11 OS lesions as indicated. The values of the detailed relative proportion of each cell cluster are provided in the Source Data file.

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